FEBS Letters · 1991 · 41 citations · 37 references
Life SciencesBiochemistryMolecular BiologyCell SignalingSignal TransductionTranscriptional RegulationCytokine BiologyCytokine SignalingGrowth Factor SignalingProtein Kinase CascadesReceptor Tyrosine Kinase SignalingTyrosine Kinase InhibitorsInositol Phosphate SignalingKinase Signaling InhibitorsPhosphoinositide 3-Kinase SignalingPhosphoinositide 3-Kinase-akt ActivationPhospholipase A2 InhibitionPhospholipase A2 PathwayProtein Tyrosine PhosphorylationTyrosine Phosphorylation Signaling
Treatment of rat mesangial cells with interleukin 1 beta (IL-1 beta) and forskolin greatly enhanced the expression of group II phospholipase A2 (PLA2) mRNA, with subsequent increased synthesis and secretion of PLA2, as detected by PLA2 activity measurements and immunoprecipitation of culture media of [35S]methionine-labelled mesangial cells. PDGF-BB dose-dependently suppressed the IL-1 beta- and forskolin-induced elevation of PLA2 mRNA, as well as PLA2 synthesis and secretion. In contrast, PDGF-AA had no inhibitory effect. The tyrosine kinase inhibitor genistein dose-dependently antagonized the inhibitory effect of PDGF-BB on IL-1 beta-stimulated PLA2 secretion, thus suggesting that tyrosine phosphorylation may be required for PDGF-BB inhibition of PLA2 gene expression in mesangial cells.
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Ulrich K. Laemmli · Nature · 1970 · 251.6K citations