FEBS Letters · 2001 · 25 citations · 10 references
Life SciencesBiochemistryMolecular BiologyProtein BiochemistryProtein-protein InteractionsProteomicsProtein Complex AnalysisProtein-ligand InteractionsProtein-protein Interaction AnalysisProtein-protein RecognitionYeast Two-hybrid Screening
By the use of the yeast two-hybrid screen we have identified two proteins that interacted with UCH37: S14, which is a subunit of PA700 and a novel protein, UIP1 (UCH37 interacting protein 1). The interaction of UCH37 with S14 or UIP1 was confirmed by in vitro binding assay and in vivo co-immunoprecipitation analysis. The C-terminal extension of UCH37 is essential for interaction with S14 or UIP1 as shown by the yeast two-hybrid assay and the in vitro binding assay. Furthermore, UIP1 blocked the interaction between UCH37 and S14 in vitro.
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Editing of ubiquitin conjugates by an isopeptidase in the 26S proteasome
Y. Amy Lam, Wei Xu, George Demartino et al. · Nature · 1997 · 456 citations