Concepedia

Using real-time PCR to determine transgene copy number in wheat

Zhiwu Li, Jennifer L. Hansen, Ying Liu, Robert S. Zemetra, P. H. Berger

Plant Molecular Biology Reporter · 2004 · 82 citations · 24 references

DOI

Concepts

References

24

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method

Kenneth J. Livak, Thomas D. Schmittgen · Methods · 2001 · 181.5K citations

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Real time quantitative PCR.

Chris Heid, Junko Stevens, Kenneth J. Livak et al. · Genome Research · 1996 · 6.2K citations · Full text

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Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.

Pamela M. Holland, Richard D. Abramson, R. R. Watson et al. · Proceedings of the National Academy of Sciences · 1991 · 2.7K citations

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Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions

Russell Higuchi, Carita Fockler, Gavin Dollinger et al. · Nature Biotechnology · 1993 · 2.2K citations

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Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.

Kenneth J. Livak, Samantha Flood, J Marmaro et al. · Genome Research · 1995 · 1.6K citations · Full text

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