Proceedings of the National Academy of Sciences · 2004 · 34 citations · 29 references
Life SciencesBiochemistryMolecular BiologyDna RepairProtein EngineeringRecombinationDna Double-strand Break RepairDna Strand BreaksDna Strand ExchangeDouble-strand Break RepairHomologous RecombinationRecombination ProteinsDna Double-strand BreaksDna Strand Break RepairDouble-strand Dna BreaksHomologous Recombination RepairOligonucleotide AnnealingRad52-mediated RepairRecombinase ProteinsStrand Exchange
Repair of double-strand breaks is essential for the maintenance of genome integrity and cell survival. In eukaryotes, double-strand-break repair by homologous recombination requires the Rad52 group of proteins. Human Rad52 protein (HsRad52)-mediated annealing of complementary strands has been studied in detail, but little has been reported on the recombinase activities of HsRad52. For this study, we purified HsRad52 from Escherichia coli. DNase I protection experiments indicated that HsRad52 binds preferentially to single-stranded DNA and protects it against digestion by DNase I. HsRad52 catalyzed D-loop formation in superhelical DNA, as well as strand exchange among oligonucleotide substrates. The formation of a stoichiometric complex between HsRad52 and single-stranded DNA was found to be critical for strand exchange activity, and the coating of both the single- and double-stranded oligonucleotides inhibited the exchange reaction.
29
Tomohiko Sugiyama, James H. New, Stephen C. Kowalczykowski · Proceedings of the National Academy of Sciences · 1998 · 311 citations · Full text
Binding of double-strand breaks in DNA by human Rad52 protein
Eric Van Dyck, Alicja Z. Stasiak, Andrzej Stasiak et al. · Nature · 1999 · 311 citations