Phosphate metabolite imaging and concentration measurements in human heart by nuclear magnetic resonance

Abstract

Cardiac-gated phosphorus (31P) nuclear magnetic resonance (NMR) spectroscopic imaging with surface coils resolves in three dimensions the spatial distribution of high energy phosphate metabolites in the human heart noninvasively. 31P spectra derive from 6- to 14-cm3 volumes of myocardium in the anterior left ventricle, septum, and apex, at depths of up to about 8 cm from the chest, as identified by proton (1H) NMR anatomical images acquired without moving the subject. Spectroscopic images are acquired in 9 to 21 min at 1.5 T. Metabolite concentrations are quantified with reference to a standard located outside the chest, yielding normal in vivo concentrations of phosphocreatine and adenosine triphosphate of about 11.0 +/- 2.7 (SD) and 6.9 +/- 1.6 mumol/g of wet heart tissue, respectively. High energy phosphate contents did not vary significantly with location in the normal myocardium, but 2,3-diphosphoglycerate signals from blood varied with subject and location.