Semisynthesis of a Glycosylphosphatidylinositol‐Anchored Prion Protein

Abstract

Pinning down the role of the anchor: The chemical synthesis of a cysteine-modified glycosylphosphatidylinositol (GPI) anchor provides access to homogeneous GPI-anchored prion protein through expressed protein ligation (see scheme). By this method, it should be possible to investigate the influence of the complex posttranslational GPI modification on protein structure and function. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2008/z802161_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.