Proceedings of the National Academy of Sciences · 1972 · 293 citations · 17 references
Natural SciencesBiochemistryBiophysicsBiophysical ChemistryMolecular BiophysicsProtein BiochemistryFluorescence SpectroscopyProtein ChemistryChromophore PhotochemistryChromophore-protein InteractionsEnergy TransferLysozyme ActivityProtein FluorescenceTryptophan Oxidation
The bulk of the fluorescence of lysozyme is located in Trp 62 and Trp 108. By examination of the fluorescence of derivatives in which Trp 62 and/or Trp 108 are specifically oxidized, it has been possible to detect a pH-dependent interaction between tryptophan residues. This interaction is interpreted as energy transfer from Trp 108 to Trp 62.
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