Ultrastructural and immunohistochemical study of the basal apparatus of solitary cilia in the human oviduct epithelium

Abstract

The basal apparatus of the solitary cilium is composed of the basal body, an associated centriole and the basal body-associated structures. To see the connection between the basal body and the centriole, we studied the basal apparatus of solitary cilia in human oviductal secretory cells by electron microscopy and immunohistochemistry. A single centriole was present in the vicinity of the basal body of a solitary cilium. The basal body and the single centriole were interconnected by one or two bundles of thin filaments with a few periodic striations. We have called these bundles the striated connector. The periodicity of striations in the striated connector measured 55 +/- 6 nm, about 15 nm shorter than that of striated rootlets. The striated connector was immunolabelled with R67 antibody specific to striated rootlets, indicating that they are composed of common molecule(s). Although the true function of the connector is unknown as yet, it could play an important role for stabilising the basal body in the apical cytoplasm.