Multiprotein Immunoassay Arrays Fabricated by Microcontact Printing

Abstract

Microarrays containing up to three different proteins were fabricated by microcontact printing (μCP) techniques and tested as a detection system for specific antibodies. After fabrication, immunoassays were successfully performed using the patterned protein microarrays. The developed immunoassays were characterized by fluorescence microscopy and scanning probe microscopy. The characterization revealed the quality of the protein deposition and indicated a high degree of selectivity for the targeted antigen−antibody interactions. The results of this study suggest that μCP is an inexpensive and effective way to fabricate biologically active substrates that can be of use for multiple reagentless immunosensor applications.