Random Walks to Synthetic Riboswitches—A High‐Throughput Selection Based on Cell Motility

Abstract

Flip the switch. Riboswitches, which are RNA sequences that undergo a ligand-induced conformational change to alter gene expression, can be used to reprogram how bacteria respond to small molecules. Here we present a new selection method based on cell motility (outlined in the figure) that rapidly identifies synthetic riboswitches and minimizes cost and effort. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2008/z700546_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.