Time resolved fluorescence of aqueous tryptophan

Abstract

The fluorescence decay of aqueous tryptophan is shown to be described by a two exponential decay function whose components have life-times of 3.14 ns and 0.51 ns. These components are assigned as the solvent equilibrated 1 L a and 1 L b , states respectively. The time-resolved emission spectra are presented and can be resolved into two spectra with λ max at 350 nm and 335 nm corresponding to these two states.