Characterization of the Functional Domains of Escherichia coli RNase II

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.1K citations

Genetics, Puc19 Vectors, Bacteriophage +9

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

F. William Studier, Barbara A. Moffatt · Journal of Molecular Biology · 1986 · 6.4K citations

Selective High-level Expression, Natural Sciences, Phage Biology +8

Amino acid substitution matrices from protein blocks.

Steven Henikoff, Jorja G. Henikoff · Proceedings of the National Academy of Sciences · 1992 · 6.3K citations · Full text

Genetics, Molecular Biology, Genomics +19

CLUSTAL: a package for performing multiple sequence alignment on a microcomputer

Desmond G. Higgins, Paul M. Sharp · Gene · 1988 · 3.4K citations

Bioinformatics, Long-read Sequencing, Natural Sciences +11

Enhanced genome annotation using structural profiles in the program 3D-PSSM 1 1Edited by J. Thornton

Lawrence A. Kelley, Robert M. MacCallum, Michael J.E. Sternberg · Journal of Molecular Biology · 2000 · 1.5K citations

Functional Genomics, Structural Profiles, Bioinformatics Database +14