Science · 2012 · 76 citations · 37 references
GeneticsGenomic MechanismMolecular BiologyMolecular GeneticsDrosophila RecMcm ComplexMeiosisDna ReplicationGameteMeiotic CrossoversChromosomal RearrangementChromatin FunctionBiologyChromatinNatural SciencesGenetic MechanismChromosome BiologyBlm HelicaseFlies DrosophilaMedicine
Generation of meiotic crossovers in many eukaryotes requires the elimination of anti-crossover activities by using the Msh4-Msh5 heterodimer to block helicases. Msh4 and Msh5 have been lost from the flies Drosophila and Glossina, but we identified a complex of minichromosome maintenance (MCM) proteins that functionally replace Msh4-Msh5. We found that REC, an ortholog of MCM8 that evolved under strong positive selection in flies, interacts with MEI-217 and MEI-218, which arose from a previously undescribed metazoan-specific MCM protein. Meiotic crossovers were reduced in Drosophila rec, mei-217, and mei-218 mutants; however, removal of the Bloom syndrome helicase (BLM) ortholog restored crossovers. Thus, MCMs were co-opted into a novel complex that replaced the meiotic pro-crossover function of Msh4-Msh5 in flies.
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MUSCLE: multiple sequence alignment with high accuracy and high throughput
R. C. Edgar · Nucleic Acids Research · 2004 · 45.3K citations · Full text
Koichiro Tamura, Daniel G. Peterson, Nora Peterson et al. · Molecular Biology and Evolution · 2011 · 40.1K citations · Full text
Maximum Parsimony Methods, Comparative Genomics, Genetics +20
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The Genome Sequence of <i>Drosophila melanogaster</i>
Mark D. Adams, S Celniker, Robert A. Holt et al. · Science · 2000 · 6K citations