Journal of Cellular Biochemistry · 1985 · 205 citations · 21 references
Life SciencesCell BiologyDevelopmental BiologyDevelopmental Cell BiologyOrganogenesisTissue DifferentiationAngiogenesisCardiovascular DevelopmentCorneal VascularizationGrowth Factor SignalingVascular DevelopmentVascular TissueAngiogenesis In NeoplasmsCation-exchange ChromatographyChorioallantoic Membrane DevelopmentCorneal AngiogenesisGrowth Factor-mediated VascularizationHeparin Affinity ChromatographySpecific Activity DeterminationVascular Endothelial Growth Factor
A growth factor mitogenic for BALB/C 3T3 cells and capillary endothelial cells was isolated from a rat chondrosarcoma and purified to homogeneity. Purification was accomplished by a combination of BioRex 70 cation exchange chromatography and heparin affinity chromatography. The pure chondrosarcoma-derived growth factor (ChDGF) had a molecular weight of about 18,000. The angiogenesis activity of pure ChDGF was tested by measuring its ability to vascularize the chorioallantoic membrane (CAM) and yolk sac membrane of the developing chick. The ability of ChDGF to induce the growth of limbal vessels in the rat cornea was also measured. To quantitate the angiogenesis response, a unit system based on the growth factor activity of ChDGF for 3T3 cells was adopted. ChDGF was found to have a specific activity of about 5 units/ng when applied to 3T3 cells. About 300-600 units of ChDGF in the two types of developing chick membrane and 30-5 units of ChDGF in the rat cornea were found to stimulate noninflammatory angiogenesis.
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Polymers for the sustained release of proteins and other macromolecules
Róbert Langer, Judah Folkman · Nature · 1976 · 1.2K citations
Heparin Affinity: Purification of a Tumor-Derived Capillary Endothelial Cell Growth Factor
Yuen Shing, Judah Folkman, Robert Sullivan et al. · Science · 1984 · 936 citations
Denis Gospodarowicz, Jinhua Cheng, G M Lui et al. · Proceedings of the National Academy of Sciences · 1984 · 622 citations · Full text