Development of a PCR method for mycoplasma testing of Chinese hamster ovary cell cultures used in the manufacture of recombinant therapeutic proteins

High and Low Annealing Temperatures Increase Both Specificity and Yield in Touchdown and Stepdown PCR

Karl Hecker, Kenneth H. Roux · BioTechniques · 1996 · 413 citations · Full text

Natural Sciences, Chemistry, Chemical Kinetics +12

Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR

F J van Kuppeveld, Karl‐Erik Johansson, J M Galama et al. · Applied and Environmental Microbiology · 1994 · 185 citations · Full text

Life Sciences, Microbiology, Molecular Biology +16

Elimination of contaminating DNA within polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens

A Meier, David H. Persing, Marion Finken et al. · Journal of Clinical Microbiology · 1993 · 173 citations · Full text

Life Sciences, Microbiology, Molecular Biology +17

A polymerase chain reaction based method for detecting Mycoplasma/Acholeplasma contaminants in cell culture

Jane Tang, Mengdong Hu, S Lee et al. · Journal of Microbiological Methods · 2000 · 129 citations

Veterinary Medicine, Infectious Diseases, Microbiology +12

Detection and tentative identification of dominant mycoplasma species in cell cultures by restriction analysis of the 16S-23S rRNA intergenic spacer regions

Ryô Harasawa, Hiroshi Mizusawa, K Nozawa et al. · Research in Microbiology · 1993 · 104 citations

Life Sciences, Microbiology, Molecular Biology +8