MC-12, an Annexin A1-Based Peptide, Is Effective in the Treatment of Experimental Colitis

Abstract

Annexin A1 (ANXA1) inhibits NF-kB, a key regulator of inflammation, the common pathophysiological mechanism of inflammatory bowel diseases (IBD). MC-12, an ANXA1-based tripeptide, suppresses NF-kB activation. Here, we determined the efficacy of MC-12 in the control of IBD. Mice with colitis induced by dextran sodium sulfate (DSS) or 2,4,6-trinitro benzene sulfonic acid (TNBS) were treated with various doses of MC-12 administered intraperitoneally, orally or intrarectally. We determined colon length and the histological score of colitis, and assayed: in colon tissue the levels of TNF-a, IFN-c, IL-1b, IL-6 and IL-10 by RT-PCR; prostaglandin E 2 (PGE 2 ), cytoplasmic phospholipase A 2 (cPLA 2 ) and myeloperoxidase by immunoassay; and COX-2 and NF-kB by immunohistochemistry; and in serum the levels of various cytokines by immunoassay. In both models MC-12: reversed dose-dependently colonic inflammation; inhibited by up to 47% myeloperoxidase activity; had a minimal effect on cytoplasmic phospholipase A 2 ; reduced significantly the induced levels of TNF-a, IFN-c, IL-1b, IL-6 and IL-10, returning them to baseline. DSS and TNBS markedly activated NF-kB in colonic epithelial cells and MC-12 decreased this effect by 85.8% and 72.5%, respectively. MC-12 had a similar effect in cultured NCM460 normal colon epithelial cells. Finally, MC-12 suppressed the induction of COX-2 expression, the level of PGE 2 in the colon and PGE 2 metabolite in serum. In conclusion, MC-12, representing a novel class of short peptide inhibitors of NF-kB, has a strong effect against colitis in two preclinical models recapitulating features of human IBD. Its mechanism of action is complex and includes pronounced inhibition of NF-kB. MC-12 merits further development as an agent for the control of IBD.