Rapid Scanning Constant Energy Synchronous Fluorescence Spectrometry: A Comparison of Flow Cells

Abstract

Rapid scanning constant energy synchronous fluorescence spectrometry (RSCESFS) is evaluated for flow cell design, scan speed, and constant energy interval choice. Analytical figures of merit for several polycyclic aromatic hydrocarbons (PAHs) are given for several experimental conditions, for several flow cells, and for both RSCESFS and conventional (nonscanning) fluorescence spectrometry. With RSCESFS, absolute detection limits as low as 700 fg, linear dynamic ranges as great as five orders of magnitude and the time to record a complete 350 nm spectrum as short as 17 s have been obtained.