Biology of the Cell · 1987 · 49 citations · 0 references
Tissue EngineeringEngineeringMultifunctional BiomaterialsSynthetic Granular HydroxyapatiteBiomaterials DesignPathologyBiomedical EngineeringOsteoporosisCellular PhysiologyBioactive MaterialRegenerative MedicineCalcium Phosphate BiomaterialsBiomaterial ModelingBioceramicCalcium IonsCell BiologyHap ParticlesVitro EffectsFibroblastic Cell BehaviorHydroxyapatiteTissue CultureMedicineBiomaterialsBiocompatible MaterialExtracellular Matrix
The effect of synthetic granular hydroxyapatite (HAP) on cultured fibroblastic cells (L929, human bone and gingiva cells) was studied. Phagocytosis of HAP particles and resulting morphological cell changes were demonstrated by microscopic examinations. Cell counts and [3H]thymidine uptake indicated significant increases in cell proliferation and DNA synthesis. These results could account for some of the alterations of the fibroblast behavior induced by changes in intracellular levels of calcium ions released from the material.