High‐Content Peptide Microarrays for Deciphering Kinase Specificity and Biology

Abstract

Thousands of kinase assays at one stroke are possible by using high-content peptide microarrays. Such experiments deliver information regarding peptidic kinase substrates and accelerate the development of high-throughput screening assays. Moreover, knowledge-based selection of human sequences allows the reliable detection of downstream targets of protein kinases, as demonstrated for 3-phosphoinositide-dependent protein kinase.