Antimicrobial Agents and Chemotherapy · 1994 · 46 citations · 24 references
Life SciencesGeneticsMolecular GeneticsEnzymologyGenomicsProtein TranslationPlasmid BiologyPlasmid GeneticsPlasmid GenomicsProtein SynthesisRecombinant Plasmid ConstructionTranslation In ProkaryotesBacterial Protein SynthesisCodingCoding Region VariationPlasmid Sequence AnalysisPlasmid SequencingProkaryotic Translation Initiation FactorsStart Codon ContextTranslation Initiation Codon Selection
The nucleotide sequence of gene aadA2 of plasmid pSa, coding for aminoglycoside-3"-adenyltransferase, has been reexamined. We found differences with respect to the sequence previously determined by Tait et al. (R. C. Tait, H. Rempel, R. L. Rodriguez, and C. I. Kado, Gene 36:97-104, 1985). These deviations are located in the coding region and in the 3' noncoding region. By making deletions in the region for initiation of protein synthesis, we identified a GTG triplet as the most probable start codon for translation.
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