The cytoplasmic domain of the T cell receptor ζ chain is sufficient to couple to receptor-associated signal transduction pathways

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays

Tim R. Mosmann · Journal of Immunological Methods · 1983 · 55.6K citations

Life Sciences, Cell Biology, Apoptosis +14

A new generation of Ca2+ indicators with greatly improved fluorescence properties.

Grzegorz Grynkiewicz, Martin Poenie, Roger Y. Tsien · Journal of Biological Chemistry · 1985 · 21.7K citations · Full text

Natural Sciences, Biochemistry, Chemistry +17

Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction

Kary B. Mullis, Fred Faloona, S. Scharf et al. · Cold Spring Harbor Symposia on Quantitative Biology · 1986 · 3.5K citations

Life Sciences, Biotechnology, Molecular Biology +15

Contingent Genetic Regulatory Events in T Lymphocyte Activation

Gerald R. Crabtree · Science · 1989 · 1.1K citations

Life Sciences, Genetics, Immunology +16