An apparent increase in symplastic water contributes to greater turgor in mycorrhizal roots of droughted <i>Rosa</i> plants*

Abstract

Using psychrometric pressure-volume analysis, root water relations following drought were characterized in Rosa hybrida L. plants colonized by the vesicular-arbuscular mycorrhizal fungus Glomus intraradices Schenck & Smith. Measurements were also made on uncolonized plants of similar size and adequate phosphorus nutrition. Under well-watered conditions mycorrhizal colonization resulted in lower solute concentrations in root symplasm, and hence lower root turgors. Following drought, however, mycorrhizal roots maintained greater turgor across a range of tissue hydration. This effect was apparently not due to increased osmotic adjustment (osmotic potentials at full turgor were similar in mycorrhizal and non-mycorrhizal roots after drought) or to altered elasticity but to an increased partitioning of water into the symplast. Symplast osmolality at full turgor was similar in mycorrhizal and non-mycorrhizal roots but, because of their higher symplastic water percentages, mycorrhizal roots contained a greater amount of osmotic (symplastic) solutes. Drought-induced changes in osmotic potential were observed only in mycorrhizal roots, where a 0.4 MPa decrease (relative to well-watered controls) brought the full turgor osmotic potential of mycorrhizal roots to the same level as that of non-mycorrhizal roots under either watering treatment.