Time-Resolved Fluorescence Microscopy Using Multichannel Photon Counting

Abstract

A new measurement system is reported which combines fluorescence microscopy with multichannel photon-counting detection. The prototype instrument can be operated in normal fluorescence and in fluorescence lifetime modes. Multichannel photon detection was used in order to enhance the rate of data collection. The feasibility of rapid two-dimensional mapping of samples was indicated. The technique may prove especially valuable in situations where multicomponent samples display similar spectral features but differing lifetime characteristics. By increases in the measurement dimensionality, enhanced sample evaluation is possible. Applications involving model and practical biological systems are discussed.