Separation of Cytokinins from Danish Cabbage by Column Chromatography on Insoluble Polyvinylpyrrolidone

Abstract

Abstract Chromatography of partially purified extracts of Danish cabbage ( Brassica oleracea var. capitata L.) heads on polyvinylpyrrolidone columns using a weak phosphate buffer indicated a cytokinin complex containing nine components active in the modified Amaranthus betacyanin bioassay. Similar elution patterns were obtained with extracts of four different cabbage varieties but varietal differences occurred in the biological activity from each component peak. Paper and thin‐layer chromatography indicated the presence of both zeatin and zeatin riboside in the extracts but the other cytokinin‐active components were not identified. Separation of sweet‐corn extracts by this technique indicated that most of the cytokinin activity occurred in the elution volume where zeatin and zeatin riboside would normally occur.