FEMS Microbiology Letters · 2006 · 32 citations · 16 references
Life SciencesMicrobiologyMolecular BiologyCell SignalingHost-pathogen InteractionsSignal TransductionBacterial PathogenesisCell Signaling NetworksInnate Immune ActivationInnate Immune Signaling PathwaysProtein Kinase CascadesProtein Phosphatase SignalingExtracellular Signal-regulated Kinase PathwayMap KinasesMapk SignalingMitogen-activated Protein Kinase CascadesMitogen-activated Protein Kinase Pathway ActivationMitogen-activated Protein Kinase PathwaysMitogen-activated Protein Kinase SignalingRaf-mek-erk Signaling
In this study we investigated the effect of Listeria monocytogenes infection on the activation of the Raf-MEK-MAP kinase pathway in eukaryotic host cells. HeLa cell infection with L. monocytogenes EGD resulted in a rapid, but transient, phosphorylation of the MAP kinases erk-1 and erk-2, a transient phosphorylation of the MAP kinase kinase MEK-1, and a transient activation of the MAP kinase kinase kinase Raf. In parallel to the transient phosphorylation of the MAP kinases, we detected induced expression of the MAP kinase phosphatase MKP-1. Additionally we present evidence that listeriolysin O is the inducing agent for activation of the Raf-MEK-MAP kinase pathway.
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