Promoter constructions for efficient secretion expression in Streptomyces lividans

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.1K citations · Full text

Dna, Engineering, Dna Analysis +20

Detection of specific sequences among DNA fragments separated by gel electrophoresis

E.M. Southern · Journal of Molecular Biology · 1975 · 32.8K citations

Chromatin, Dna Sequencing, Dna +15

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.1K citations

Genetics, Puc19 Vectors, Bacteriophage +9

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

Hermann Schägger, Gebhard von Jagow · Analytical Biochemistry · 1987 · 11.5K citations

Protein Chemistry, Biochemistry, Natural Sciences +8

Genomic sequencing.

George M. Church, W Gilbert · Proceedings of the National Academy of Sciences · 1984 · 8.2K citations · Full text

Dna Sequencing, Dna, Molecular Biological Method +13