An improved Tn7-based system for the single-copy insertion of cloned genes into chromosomes of gram-negative bacteria

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.2K citations

Life Sciences, Microbiology, Molecular Biology +11

A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria

Reinhard Simon, U. B. Priefer, Alfred Pühler · Bio/Technology · 1983 · 7.3K citations

Life Sciences, Genetics, Molecular Genetics +12

A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR

Virginia L. Miller, John J. Mekalanos · Journal of Bacteriology · 1988 · 2.1K citations · Full text

Life Sciences, Genetics, Molecular Biology +17

Tn7 transposition: two transposition pathways directed by five Tn7-encoded genes.

C S Waddell, Nancy L. Craig · Genes & Development · 1988 · 128 citations · Full text

Life Sciences, Genetics, Molecular Genetics +12

A broad-host-range cloning vector transposable to various replicons

Nigel J. Grinter · Gene · 1983 · 97 citations

Life Sciences, Genetics, Molecular Genetics +14