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An improved Tn7-based system for the single-copy insertion of cloned genes into chromosomes of gram-negative bacteria

EngineeringVirulence FactorGeneticsTn7-based SystemBacteriologySingle-copy InsertionSynthetic BiologyMolecular BiologyGenetic EngineeringGram-negative BacteriaMicrobiologyMolecular MicrobiologyMedicineCloningMicrobial Genetics