Generation and Enzymatic Amplification of High‐Density Functionalized DNA Double Strands

Abstract

Up to eight modifications could be incorporated into a DNA heteroduplex (see picture) by introducing different sets of 2′-deoxynucleotide derivatives through DNA polymerase mediated primer extension. All four natural nucleobases in each strand were substituted with different base-modified analogues. PCR conditions are described that allow the direct amplification of fully functionalized DNA double strands. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2004/z53926_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.