Antibody‐Free Detection of Protein Tyrosine Nitration in Tissue Sections

Abstract

Inflamed proteins: Tyrosine nitration is a covalent post-translational protein modification that represents a biomarker for inflammatory diseases. We introduce a novel method to detect nitrotyrosine in biological samples by chemoselective conversion into a fluorophore. We describe the applicability of this methodology to detect protein-bound nitrotyrosine by fluorescence microscopy in tissue sections and on Western blot membranes. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.