Engineering Resistance to Mixed Virus Infection in a Commercial Potato Cultivar: Resistance to Potato Virus X and Potato Virus Y in Transgenic Russet Burbank

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

Thomas A. Kunkel, John D. Roberts, Richard A. Zakour · Methods in enzymology on CD-ROM/Methods in enzymology · 1987 · 5.3K citations

Life Sciences, Biotechnology, Molecular Biology +8

Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.

Gary S. Ditta, S W Stanfield, Donald R. Helinski · Proceedings of the National Academy of Sciences · 1980 · 2.7K citations · Full text

Life Sciences, Genetics, Microbiology +17

Delay of Disease Development in Transgenic Plants That Express the Tobacco Mosaic Virus Coat Protein Gene

Patricia Abel, Richard S. Nelson, Barun K. De et al. · Science · 1986 · 1.3K citations

Agriculture, Plant Biology, Plant Sciences +17

Duplication of CaMV 35 <i>S</i> Promoter Sequences Creates a Strong Enhancer for Plant Genes

Robert J. Kay, Amy M. Chan, Mark Daly et al. · Science · 1987 · 896 citations

Life Sciences, Genetics, Plant Biology +17