Amperometric Methods for Oxidoreductase Enzymes Based on Liquid Chromatography with Electrochemical Detection. Alcohol Dehydrogenase

Abstract

Abstract Nicotinamide adenine dinucleotide (NAD) is an important cofactor in a number of oxidoreductase enzyme systems. The detection and quantitation of its reduced form (NADH) is the basis for a number of methods which determine both substrates and enzyme activity. Although electrochemical techniques have shown promise in this area, they have suffered from a lack of selectivity. Liquid chromatography with electrochemical detection (LCEC) provides the selectivity and the sensitivity required. The use of alcohol dehydrogenase (ADH) for blood alcohol detection is investigated as a model system.