Proceedings of the National Academy of Sciences · 1979 · 87 citations · 19 references
Amino AcidsCytogeneticsGeneticsGenetic EpidemiologyHuman PolymorphismMolecular BiologyImmunophenotypingCell CultureMolecular GeneticsClinical GeneticsHuman PhenotypesMolecular EcologyHuman VariationGenotype-phenotype AssociationPublic HealthProteomicsHuman HeterozygosityMolecular Biological MethodHistopathologyStatistical GeneticsGene ProductsPopulation GeneticsCell BiologyAmino AcidEvolutionary BiologyMedicine
Several hundred polypeptides from four human diploid fibroblast cell lines were compared by high-resolution two-dimensional polyacrylamide gel electrophoresis and double-label autoradiography under conditions where allelic products that differ by a single charged amino acid would be distinguished. The average heterozygosity represented by this set of gene products appears to be less than 1% for changes involving charged amino acids.
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High resolution two-dimensional electrophoresis of proteins.
Patrick H. O’Farrell · Journal of Biological Chemistry · 1975 · 19.3K citations · Full text
E. Kaltschmidt, H. G. Wittmann · Analytical Biochemistry · 1970 · 1.1K citations
Ribosomal Proteins, Protein Biosynthesis, Molecular Biology +4