A Laser Temperature-Jump Apparatus With Pulsed Spectrophotometric Detection Application To The Study Of Conformational Equilibria Of Adenyl 3′ 5′ Adenosine¹

Abstract

ABSTRACT A Raman laser temperature-jump apparatus with highly sensitive (better than 10 −3 O.D. units) and very fast (rise time about 10 ns) pulsed spectrophotometric detection is described. A temperature jump of over 6°C is achieved within 20 ns in a special variable-path flow microcell. To illustrate the capabilities of the apparatus the kinetics of the conformation change of adenylyl 3‘→5’ adenosine in aqueous solution has been studied.