International Journal of Molecular Sciences · 2015 · 29 citations · 19 references
Life SciencesCell BiologyOphthalmologyApoptosisAutophagyCell SignalingApoptosis ModulationApoptosis RegulationApoptosis ResistanceApoptotic SignalingRetinal DegenerationRetinal Pigment EpitheliumAnimal Model EvaluationAnti-apoptotic AgentsAnti-apoptotic ModulationEndoplasmic Reticulum Stress ResponsesRetinal NeuroprotectionRetinal Pigment Epithelium Cell DeathUnfolded Protein ResponseWestern Blot Analysis
BACKGROUND: Studies have shown that mini-αA can protect retinal pigment epithelium (RPE) cells from apoptosis. However, no in vivo study concerning the anti-apoptotic function of mini-αA has been conducted yet. METHODS: MTT assay, HE staining and TUNEL assay were used to assess levels of cells, and an animal model was established to examine the protective effects of mini-αA against NaIO3-induced RPE cell apoptosis. Western blot analysis and RT-qPCR were performed to explore the possible mechanism of mini-αA's protective function against NaIO3-induced RPE cell apoptosis. RESULTS: RESULTS from in vivo and animal experiments showed that mini-αA antagonized NaIO3-induced RPE cell apoptosis. Further investigation into how mini-αA provided protection against NaIO3-induced RPE cell apoptosis showed that mini-αA reduced NaIO3-induced RPE cell apoptosis and autophagy. In addition, unfolded protein response was also involved in the protective effects of mini-αA against NaIO3-induced RPE cell apoptosis. CONCLUSIONS: mini-αA can antagonize RPE cell apoptosis induced by NaIO3. A possible mechanism is by inhibition of apoptosis by repressing autophagy and endoplasmic reticulum stress.
19
Kanefusa Kato, Haruo Shinohara, Naomi Kurobe et al. · Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology · 1991 · 217 citations