New Automated Method for Determining Postantifungal Effect of Amphotericin B against<i>Candida</i>Species: Effects of Concentration, Exposure Time, and Area under the Curve

Abstract

The postantifungal effect (PAFE) of amphotericin B was determined with a BacT/Alert automated system, which is based on the colorimetric detection of CO(2). The levels of accuracy and precision of the automated method were high. Longer PAFEs were obtained for Candida albicans (P < 0.001), C. glabrata (P < 0.01), and C. krusei (P < 0.01) at increasing amphotericin B concentrations and exposure times.