Concepedia

Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of <i>Vibrio cholerae</i> O1 biotype El Tor

Masatomo Morita, Makoto Ohnishi, Eiji Arakawa, N. A. Bhuiyan, Suraia Nusrin, Munirul Alam, Abdullah Siddique, Firdausi Qadri, Hidemasa Izumiya, G. Balakrish Nair,

Microbiology and Immunology · 2008 · 114 citations · 12 references

DOIFull text

Open access

Concepts

Abstract

A mismatch amplification mutation PCR assay was developed and validated for rapid detection of the biotype specific cholera toxin B subunit of V. cholerae O1. This assay will enable easy monitoring of the spread of a new emerging variant of the El Tor biotype of V. cholerae O1.

References

12

Mismatch amplification mutation assay (MAMA): application to the c-H-ras gene.

Helmut Zarbl, Phouthone Keohavong, William G. Thilly · Genome Research · 1992 · 384 citations · Full text

, , +16

Identification ofEscherichia coli heat-labile enterotoxin by means of a ganglioside immunosorbent assay (GM1-ELISA) procedure

Ann‐Mari Svennerholm, Jan Holmgren · Current Microbiology · 1978 · 349 citations

, , +14

New Variants of <i>Vibrio cholerae</i> O1 Biotype El Tor with Attributes of the Classical Biotype from Hospitalized Patients with Acute Diarrhea in Bangladesh

G. Balakrish Nair, Shah M. Faruque, N. A. Bhuiyan et al. · Journal of Clinical Microbiology · 2002 · 288 citations · Full text

, , +15

Cholera Due to Altered El Tor Strains of <i>Vibrio cholerae</i> O1 in Bangladesh

G. Balakrish Nair, Firdausi Qadri, Jan Holmgren et al. · Journal of Clinical Microbiology · 2006 · 238 citations · Full text

, , +15

Use of automated sequencing of polymerase chain reaction-generated amplicons to identify three types of cholera toxin subunit B in Vibrio cholerae O1 strains

Johan Wahlberg, Bertil Petterson, Mathias Uhlén et al. · Journal of Clinical Microbiology · 1993 · 209 citations · Full text

, , +15