Cell Cycle · 2003 · 66 citations · 34 references
Life SciencesBiologyCell BiologyCell CycleDna RepairRecombinationDna Double-strand Break RepairDna Strand BreaksDouble-strand Break RepairHomologous RecombinationS PhaseDna Double-strand BreaksDna Repair MechanismsDna Strand Break RepairDouble-strand Break Repair PathwaysDouble-strand Dna BreaksHomologous Recombination RepairRad52-mediated RepairYeast Dna Repair
In eukaryotes, homologous recombination is an important pathway for the repair of DNA double-strand breaks. We have studied this process in living cells in the yeast Saccharomyces cerevisiae using Rad52 as a cell biological marker. In response to DNA damage, Rad52 redistributes itself and forms foci specifically during S phase. We have shown previously that Rad52 foci are centers of DNA repair where multiple DNA double-strand breaks colocalize. Here we report a correlation between the timing of Rad52 focus formation and modification of the Rad52 protein. In addition, we show that the two ends of a double-strand break are held tightly together in the majority of cells. Interestingly, in a small but significant fraction of the S phase cells, the two ends of a break separate suggesting that mechanisms exist to reassociate and align these ends for proper DNA repair.
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A monomeric red fluorescent protein
Robert E. Campbell, Oded Tour, Amy E. Palmer et al. · Proceedings of the National Academy of Sciences · 2002 · 2.4K citations · Full text
Planning, Environmental Sciences, Infrastructure Planning +14
Crystal Structure of the <b> <i>Aequorea victoria</i> </b> Green Fluorescent Protein
Mats Ormö, A B Cubitt, Karen Kallio et al. · Science · 1996 · 2.2K citations