New RP-UFLC Method for Estimation of Xanthinol Nicotinate in Pharmaceutical Dosage Form

Abstract

A novel, accurate and precise RP-UFLC method for determination of xanthinol nicotinate has been developed and validated.Separation was achieved on an Enable C 18 G column (250 mm × 4.6 mm i.d., 5 μm) using acetonitrile: 10 mM TBAHS (40:60, v/v) as mobile phase at a flow rate of 0.8 mL/min and PDA detection at 274 nm.Linearity was observed in the concentration range of 1.0-60 μg/mL (r 2 =0.999).The method was validated for accuracy, precision, stability, specificity, robustness and system suitability.Forced degradation was performed by using HCl, NaOH, H 2 O 2 , thermal and UV radiation.The method was used successfully for the determination of xanthinol nicotinate in tablet dosage form.