Analytical Letters · 1976 · 28 citations · 16 references
Life SciencesBiochemistryToxicologyAnalytical ChemistryAnalytical ToxicologyEnzymologyCarbamate ToxicologyEnzymatic BiosensorsEnzyme AssaysEnzyme InhibitionInsecticide ToxicologyPesticide Residue AnalysisAnalytical Methods DevelopmentCarbamate Pesticide ToxicityCarbamate PesticidesEnzyme ActivityEnzyme Inhibition BiosensorsFluorometric Enzyme AssaysHigh-performance Liquid ChromatographyPesticide Detection
Abstract A fluorometric enzyme inhibition detector has been developed for the detection of carbamate pesticides after separation using high speed liquid chromatography. Housefly head cholinesterase was found to have the greatest sensitivity toward the carbamates, together with horse plasma and bovine erythrocyte cholinesterases. The substrate N-methyl indoxyl acetate is used for monitoring of enzyme activity.
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