Quantifying the Performance of Protein‐Resisting Surfaces at Ultra‐Low Protein Coverages using Kinesin Motor Proteins as Probes

Abstract

The binding kinetics of fluorescently labeled microtubules to kinesin-coated surfaces permits the determination of the density of kinesin motor proteins adhered to the surface in the range of 0.1 – 30 μm–2. This extreme sensitivity, corresponding to protein coverages of 0.004 – 1 ng cm–2, enables the characterization of advanced non-fouling coatings, such as (EG)3OH-terminated SAMs and PEGMA with applications in biomedical engineering and bionanotechnology.