Current Protocols in Molecular Biology · 2013 · 36 citations · 16 references
EngineeringMolecular BiologyMolecular GeneticsBioseparationsNucleic Acid BiochemistrySeparation ProcessesExtraction TechniquesIsolation ProceduresNucleic Acid ExtractionNucleic Acid PurificationDna ExtractionMolecular Diagnosis From Tissue SamplesMolecular Genetic TestingPhenol ExtractionPhenol-chloroform ExtractionSilica-based Dna ExtractionTissue Nucleic Acid ExtractionTotal Nucleic Acid Extraction
DNA isolation is a fundamental initial step for molecular genetic studies. Two quite different methodologies are described in this unit: silica spin column and phenol/chloroform extraction. Currently, the most commonly used technique is the silica-based spin column, although phenol/chloroform extraction is still widely used. This unit also presents basic procedures for extraction of DNA from both fresh tissues and formalin-fixed, paraffin-embedded (FFPE) tissue samples.
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