Efficient Enzymatic Glycosylation of Peptides and Oligosaccharides from GalNAc and UTP

Abstract

One-pot synthesis: A system has been developed to glycosylate peptides, proteins, and oligosaccharides more efficiently than conventional procedures. With the simple precursors GalNAc, UTP, and creatine-P, four enzymes are employed in one pot to regenerate UDP-GalNAc which is used for the transfer of GalNAc to specific acceptor substrates. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2007/z600369_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.