Crystallization and Structural Analysis of Cytochrome<i>c</i><sub>6</sub>from the Diatom<i>Phaeodactylum tricornutum</i>at 1.5 Å Resolution

Hideharu Akazaki, Fumihiro Kawai, Masaki HOSOKAWA, Toshiyuki Hama, Hirotaka Chida, Takako Hirano, Boon-Keng Lim, Nobuo Sakurai, Wataru Hakamata, Sam‐Yong Park,

Bioscience Biotechnology and Biochemistry · 2009 · 13 citations · 12 references

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Abstract

We determined for the first time the crystal structure of diatom cytochrome c(6) from Phaeodactylum tricornutum at 1.5 A resolution. The overall structure of the protein was classified as a class I c-type cytochrome. The physicochemical properties of the protein were examined by denaturation with guanidine hydrochloride and urea, and compared with those of other algal cytochrome c(6).

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