Publication | Closed Access
High Performance Liquid Cbromatographic Separation of Globin Chains on a Large-Pore C<sub>4</sub>Column
420
Citations
7
References
1984
Year
Baboon Globin ChainsGlycobiologyMolecular BiologyAnalytical UltracentrifugationSeparation ScienceBioanalysisBiochemical GeneticsAnalytical ChemistrySeparation TechniqueLiquid ChromatographyClinical ChemistryChromatographyGlycosylationProtein ChemistryBiochemistryBiomolecular AnalysisChromatographic AnalysisBiomolecular EngineeringAbstract Excellent ResolutionGlobin ChainsNatural SciencesNormal ChainsMedicine
Abstract Excellent resolution of human and baboon globin chains may be obtained by HPLC on a Vydac large-pore C4 column. The procedure is rapid and uses a gradient between aqueous trifluoroacetic acid and trifluoroacetic acid in acetonitrile. The common human γ chains are easily separable from each other as are some α- and β-chain variants from the normal chains and from each other.
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