Protein Purification: Adsorption Chromatography on Controlled Pore Glass with the Use of Chaotropic Buffers

Abstract

Chromatography on controlled pore glass in combination with chaotropic buffers makes possible, in a single step, protein purifications of several hundredfold. The new emphasis is on highly selective controllable adsorption. The method is useful for the purification and concentration of proteins from large volumes of complex media and for the purification of proteins that are poorly soluble or tend to aggregate in aqueous solution. D-(-)-β-Hydroxybutyrate dehydrogenase, a mitochondrial membrane-bound protein, several soluble proteins, and staphylococcal α toxin, which can be purified directly from large volumes of culture medium, are used to illustrate the method.