The Journal of General and Applied Microbiology · 1969 · 21 citations · 0 references
Life SciencesBiochemistryChemistryAnalytical ChemistryChromatographic AnalysisSteroid BiochemistryChiral ChromatographyChromatographySteroid BiosynthesisSteroid Hormone BiochemistrySteroid StructureThin-layer ChromatographyChirality In SteroidsMass Spectrometric Structure ElucidationSilica Gel TreatmentSteroid Hormone AssaysSteroid Isomerism
The resolution of 30 different C21, C19 and C18 steroids by various solvent systems on silica gel chromatoplates was reported. Effective resolution of Δ4-pregnene, 5α, axial-equatorial hydroxyl epimers and positional isomers was successfully achieved. The differentiation between these compounds by using four different colour reagents has been also reported.