The amino acid sequence of a trypsin inhibitor from the seeds of <i>Momordica charantia</i> Linn. Cucurbitaceae

Abstract

A trypsin inhibitor (MCI‐3) was isolated from the seeds of Momordica charantia Linn. Cucurbitaceae in three steps involving the affinity chromatography, CM‐Sephadex chromatography and HPLC. It is composed of 62 amino acid residues: Asp 3 Thr. 5 Ser 4 Glu 5 Pro 4 Gly 7 Ala 8 Val 9 Ile 4 Leu 1 Lys 3 Arg 8 Trp 1 ; contained no cysteine; and its M r was 7443. The amino acid sequence showed significant homology with ‘potato inhibitor I family’ inhibitors.