Concepedia

Enzymatic Glycosylation of Peptide Arrays on Gold Surfaces

Nicolas Laurent, Josef Voglmeir, Adam Wright, Jonathan M. Blackburn, Nhan T. Pham, Stephen C. C. Wong, Simon J. Gaskell, Sabine L. Flitsch

ChemBioChem · 2008 · 61 citations · 19 references

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Abstract

Play it again, SAM. Peptide substrates immobilised on self-assembled monolayer (SAM)-coated gold surfaces were efficiently glycosylated with UDP-N-acetyl-α-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (ppGalNAcT2) to form the α-D-GalNAc threonine/serine linkage. Label-free analysis of the reactions were performed by using MALDI-ToF mass spectrometry.

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