Enzymatic Glycosylation of Peptide Arrays on Gold Surfaces

Abstract

Play it again, SAM. Peptide substrates immobilised on self-assembled monolayer (SAM)-coated gold surfaces were efficiently glycosylated with UDP-N-acetyl-α-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (ppGalNAcT2) to form the α-D-GalNAc threonine/serine linkage. Label-free analysis of the reactions were performed by using MALDI-ToF mass spectrometry.