Antioxidative Effects of Glycoprotein Isolated from <i>Solanum nigrum</i> Linne on Oxygen Radicals and its Cytotoxic Effects on the MCF‐7 Cell

Abstract

ABSTRACT: Glycoprotein of Solanum nigrum Linne (SNL glycoprotein) was isolated and identified using SDS‐PAGE. SNL glycoprotein's antioxidative effects were tested in vitro and its cytotoxicity effects were tested using MCF‐7 cells. One glycoprotein was isolated from SNL fruits, the other from stems and leaves. SNL glycoprotein is reactive with oxygen radicals, such as the ‐OH and O 2 ‐ in vitro. Moreover, the SNL glycoprotein's radical scavenging activity is sensitive to the superoxide anion radical and the hydroxyl radical. In the MCF‐7 cell, SNL glycoprotein I had a cytotoxic effect at 1 μg/mL and SNL glycoprotein II at 100 μg/mL. When MCF‐7 cells were treated with SNL glycoproteins, nitrite oxide production was induced.