Generation of porcine reproductive and respiratory syndrome virus by in vitro assembly of viral genomic cDNA fragments

Enzymatic assembly of DNA molecules up to several hundred kilobases

Daniel G. Gibson, Lei Young, Ray-Yuan Chuang et al. · Nature Methods · 2009 · 10.6K citations

Dna Nanotechnology, Dna Sequencing, Dna +9

Complete Chemical Synthesis, Assembly, and Cloning of a <i>Mycoplasma genitalium</i> Genome

Daniel G. Gibson, Gwynedd A. Benders, Cynthia Andrews‐Pfannkoch et al. · Science · 2008 · 1.2K citations

Genetics, Molecular Biology, Escherichia Coli +19

Engineering the largest RNA virus genome as an infectious bacterial artificial chromosome

Fernando Almazán, José M. González, Zoltán Pénzes et al. · Proceedings of the National Academy of Sciences · 2000 · 384 citations · Full text

Synthetic Virus, Viral Replication, Engineering +14

Chemical synthesis of the mouse mitochondrial genome

Daniel G. Gibson, Hamilton O. Smith, Clyde A. Hutchison et al. · Nature Methods · 2010 · 353 citations

Biosynthesis, Mitochondrial Biogenesis, Mitochondrial Function +6

CD163 Expression Confers Susceptibility to Porcine Reproductive and Respiratory Syndrome Viruses

Jay G. Calvert, David E. Slade, Shelly Shields et al. · Journal of Virology · 2007 · 349 citations · Full text